■ 基本信息
別名: | pSilencer2.1-U6 neo |
啟動(dòng)子: | U6 |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 |
質(zhì)粒大小: | 4521bp |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | U6:ATGGACTATCATATGCTTACCGTA |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA
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片段物種: |
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原核抗性: | Amp |
真核抗性: | G418
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
This Ambion vector is for the expression of siRNA. It has an antibiotic resistance gene (neomycin) to enable selection of transfected cells and features the human RNA polymerase III promoter (U6). Sufficient reagents are provided for 20 reactions.
■ 質(zhì)粒圖譜
