■基本信息
啟動子: | 35s |
復制子: | ori |
終止子: | NOS |
質粒分類: | 植物系列，蛋白過表達載體 |
質粒大小: | 4530bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
5'測序引物: | 35S:GACGCACAATCCCACTATCC |
3'測序引物: | 根據(jù)序列設計引物 |
■質粒屬性
質粒宿主: | 植物 |
質粒用途: | 蛋白表達 |
片段類型: |
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | 綠色
|
■質粒簡介
Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.
■質粒圖譜
■質粒序列
質粒序列請下載：
ZK855pBI221-EGFP植物表達質粒.txt
