■ 基本信息
啟動(dòng)子: | TipA |
質(zhì)粒大小: | 7089bp |
質(zhì)粒標(biāo)簽: | N-6xHis/thrombin site/T7 tag，C-6xHis |
原核抗性: | Apr |
真核抗性: | Tsr |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 鏈霉菌 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 廣宿主 |
質(zhì)粒用途: |
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片段類型: |
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片段物種: |
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原核抗性: | Apr |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pGM1190是一個(gè)鏈霉菌表達(dá)載體，通過(guò)TipA 啟動(dòng)子驅(qū)動(dòng)目的基因的表達(dá)。轉(zhuǎn)化進(jìn)鏈霉素的時(shí)候請(qǐng)用35℃的溫度培養(yǎng)。OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces，replicates also in Streptomyces (below 35°C).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK1715 pGM1190鏈霉菌表達(dá)質(zhì)粒.txt
